HTTP RFC 2396 defines path separator to be single slash.
However, unless you're using some kind of URL rewriting (in which case the rewriting rules may be affected by the number of slashes), the uri maps to a path on disk, but in (most?) modern operating systems (Linux/Unix, Windows), multiple path separators in a row do not have any special meaning, so /path/to/foo and /path//to////foo would eventually map to the same file.
An additional thing that might be affected is caching. Since both your browser and the server cache individual pages (according to their caching settings), requesting same file multiple times via slightly different URIs might affect the caching (depending on server and client implementation).
与恶龙缠斗过久,自身亦成为恶龙;凝视深渊过久,深渊将回以凝视…